anti par monoclonal antibody  (R&D Systems)

Journal: Chemico-biological interactions

Article Title: Molecular and toxicological mechanisms behind the effects of chromium (VI) on the male reproductive system of Mytilus galloprovincialis: First evidence for poly-ADP-ribosylation of protamine-like II.

doi: 10.1016/j.cbi.2024.111186

Figure Lengend Snippet: Fig. 5. Western blotting performed in gonad homogenates from unexposed (Unx) and exposed M. galloprovincialis to 1 nM, 10 and 100 nM Cr(VI); (a) 12 % (SDS- PAGE); (b) anti-PARP immunoblotting; (c) anti-PAR immunoblotting; (d) densitometric analysis of 50 kDa bands immunopositive to anti-PARP; (e) densitometric analysis band immunopositive to anti-PARP with molecular weight between 30 and 40 kDa; (f) Kruskal-Wallis test followed by Dunn’s multiple comparison test was used to highlight statistically significant differences between gonad homogenates. The data are showed as mean ± SD and the minimum level of acceptable sig- nificance was set at p < 0.05.

Article Snippet: After repeated washings in Tris-buffered saline (50 mM Tris-HCl and 150 mM NaCl, pH 8.0), the immunoblotting was carried out using monoclonal anti-poly ADP-ribose (PAR) antibody (sc-56198, Santa Cruz, Biotechnology, Inc., Texas, USA, 1:500) and as anti-mouse secondary antibody was utilized horseradish peroxidase (HRP)-conjugated (sc-525409, Santa Cruz Biotechnology, Inc., Texas, USA, 1:2000).

Techniques: Western Blot, SDS Page, Molecular Weight, Comparison

Journal: Chemico-biological interactions

Article Title: Molecular and toxicological mechanisms behind the effects of chromium (VI) on the male reproductive system of Mytilus galloprovincialis: First evidence for poly-ADP-ribosylation of protamine-like II.

doi: 10.1016/j.cbi.2024.111186

Figure Lengend Snippet: Fig. 7. Western blotting carried out on PL proteins and H1 histone of M. galloprovincialis unexposed (Unx) and exposed to 1 nM, 10 and 100 nMCr(VI). (a):11.2 % acetic acid-urea polyacrylamide gel (AU-PAGE). (b): anti-PAR immunoblotting.

Article Snippet: After repeated washings in Tris-buffered saline (50 mM Tris-HCl and 150 mM NaCl, pH 8.0), the immunoblotting was carried out using monoclonal anti-poly ADP-ribose (PAR) antibody (sc-56198, Santa Cruz, Biotechnology, Inc., Texas, USA, 1:500) and as anti-mouse secondary antibody was utilized horseradish peroxidase (HRP)-conjugated (sc-525409, Santa Cruz Biotechnology, Inc., Texas, USA, 1:2000).

Techniques: Western Blot